组学样品保存液--RNA、DNA和蛋白质和细胞完整性保存液
CellCover
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产品编号： 800-050
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相关CAS号：
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分子量：
| 订货号 | 规格 | 价格 | 库存 | 数量 | 购买 | |
|---|---|---|---|---|---|---|
| 800-050 | 50ml | 1600.00元 | 1 |
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CellCover是一种即用型溶液，用于同时完全保护人类和动物实体组织中的 RNA、DNA 和蛋白质，包括肿瘤、白细胞和培养细胞（贴壁、悬浮液、球状体）。Ready to use solution for complete protecting of RNA, DNA and protein in human and animal solid tissues, including tumors, white blood cells and cultured cells (adherent, suspension, spheroids).
Anacyte Laboratories多年只专注于一款产品，就是细胞/组织原生状态保存产品CellCover， 是一款即用的，无毒的液体固定液，随着细胞与CellCover的接触，其代谢状态被即刻冻结，生物大分子的合成和转运活动立即停止；细胞内各种降解通路即时中断，化学降解同样被迅速抑制而终止；由此保证DNA、RNA和蛋白质等生物大分子的绝佳稳定性。在不需要化学交联的情况下保持细胞形状的完整性。用于保护包括肿瘤、白细胞和培养细胞（贴壁、悬浮、球状体）的RNA、DNA和蛋白质和细胞形状的完整性。
CellCover 是 唯一允许在细胞生理/病理状态环境中即刻并行存储蛋白质、RNA 和 DNA 的试剂。
* 保持细胞形状完整性，无需化学交联：在几乎任何基质上生长的细胞都可以用 CellCover 通过液体冷冻进行处理，以保持体内形态，而不会 在细胞固定过程中发生生物分子的化学交联。
* 保护 DNA，RNA 和蛋白质、蛋白质表达和基因表达状态：旨在保护人类和动物细胞和实体组织（包括肿瘤和培养细胞（贴壁细胞、悬浮细胞、球体））中 DNA、RNA 和蛋白质表达和基因表达的状态。
*一步快速稳定: CellCover 是为 生命科学研究中生物分子的快速“一步”稳定而开发的，使用 CellCover 进行 RNA 固定并充当 RNA 稳定剂，可抑制 RNA 降解，从而在 RNA 测序或单细胞 RNA seq 或单细胞测序实验中取得很好的结果（高RIN值）。
* 无毒，即用型溶液: CellCover 是一种无毒配方，是甲醛的替代品，用于人类和动物细胞和实体组织，包括肿瘤和培养细胞（贴壁细胞、悬浮细胞、球体）
* 用于基因组分析、转录组分析和蛋白质组分析的真正具有代表性的分子：CellCover 充当 DNA 和 RNA 稳定剂和屏蔽剂，例如 用于通过流式细胞术或 NGS 或 RNA seq 方法进行分析。CellCover 是基因组分析、转录组分析和蛋白质组分析成功实验的关键初始步骤。
* 几乎所有下游应用都与使用 CellCover 的细胞固定兼容，包括单细胞 RNA 测序、NGS、蛋白质测序等：
CellCover产品详情介绍Q1. Can I store my purified RNA in CellCover?
Yes, you can. Storage of RNA in CellCoverprotects your RNA sufficiently for most subsequent purposes.
Q2. How shall I store my purified RNA in CellCover?
This really depends on when do you need it. After purification you usually have it on ice. If you need it for e. g. a Northern analysis within the next two days, keep it on ice (best in a cold room for the ice lasts longer). If you are not sure when you start your subsequent analysis, freeze it at minus 20°C. However, if you want proper maintenance for several month consider storage at -80°C.
Q3. My purified RNA is partly degraded, what might have been wrong?
Degradation, usually from the 5´end, is a common problem when using purification kits. We recommend to strictly adhere to instructions of the manufacturer of the kit and to work as cold as possible (above 0°C). After precipitation, we recommend dissolving purified RNA in ice-cold CellCover.
Q4. Does my purified RNA degrade in CellCover?
RNA protection by CellCoveris almost as effective as deep freezing and sufficient for most purposes (e.g. no change in RIN after 24 h on ice).
Q5. What are typical RIN values if using CellCover?
RIN values using CellCover are generally among the best you can get. You can expect RIN values of 10 even after storing cells for days in a fridge. However, many variables influence the individual experiment, e.g. RIN value increases the more experienced the researcher is, is dependent on the kit used for purification and might differ from cell type to cell type.
Q6. In tissue, how long does it take until my RNA is protected with CellCover?
CellCover works on to ends, chemical stabilisation and protection against enzymatic degradation. Time to fully protect RNA increases in the following order: non-adherent cells- adherent cells – spheroids – tissue. As a guideline: the more cells, the longer it takes. Usually storing a 5 mm tissue block sample on ice o/n is sufficient. Store spleen or central nervous system tissue for at least 24 h on ice.
Q7. Can I freeze my tissue samples in CellCover?
You can freeze your sample in CellCover, if protein in your sample is of interest for you. However, you should leave your sample on ice o/n for some time before freezing.
Q8. Is there something important to know when isolating RNA from tissue in CellCover?
Any manipulation might affect your RNA. Cell lysis is one of the most critical steps for it must be fast and efficient. Cells can be resuspended in a small volume CellCover before adding Lysis Buffer. Tissue should be minced on ice or grind under liquid nitrogen before adding Lysis Buffer.
Q9. Can I analyse my cells in CellCover with MALDI?
Yes, you can. Follow your lab protocol. Ingredients of CellCover do not interfere with macromolecular analyses.
Q10. Does CellCover protect HMW-RNA?
CellCover protects5´prime ends of RNA , so you can analyse large RNA species more easily. Some 20 kbases should not be a problem.
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