生物素-Ahx-Ub-VME
Biotin-Ahx-Ub-VME
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产品编号： UbiQ-054
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相关CAS号：
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分子式：
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分子量：8945 Da
| 订货号 | 规格 | 价格 | 库存 | 数量 | 购买 | |
|---|---|---|---|---|---|---|
| UbiQ-054 | 50ug | 6000.00元 | 2 |
|
UbiQ-054（Biotin-Ahx-Ub-VME）是一种有效，不可逆和特异性的去泛素化酶抑制剂，用生物素标记在N末端。氨基己酸接头用于在生物素和Ub蛋白之间创造额外的空间，以便有效地获得生物素结合实体。
UbiQ-054 (Biotin-Ahx-Ub-VME) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes, which is labeled on the N-terminus with biotin. An aminohexanoic acid linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities.
品名：Biotin-Ahx-Ub-VME (human sequence, synthetic)
货号 : UbiQ-054
规格 : 50 ug, lyophilized powder
纯度 : ≥95% by cation-chromatography and RP-HPLC
分子量 : found 8944 Da, calc 8945 Da
存储 : powder at −20°C, solution at −80°C. Please avoid multiple freeze/thaw cycles.
序列：Biotin-Ahx-MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG-VME
1. Misaghi, S., et al. Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate. J. Biol. Chem. 280, 1512-1520 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15531586
2. de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887
说明书下载：
UbiQ-054说明书
样品制备注意事项：
• dissolve the powder in as little DMSO as possible (20 - 40 mg/mL)
• add the DMSO stock to milliQ (please note the order of addition) and mix
• buffer the aq. solution as desired (using 1M HEPES or 1M Tris for example)
• in general, DMSO concentrations up to 5 vol% are well tolerated by most enzymes.
• If required, total removal of DMSO is accomplished by dialysis or spin-filtration (3 kDa cut-off membrane).
• For detailed experimental conditions please see the open-access reference 1: http://onlinelibrary.wiley.com/doi/10.1002/cbic.201200497/abstract
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