悬浮细胞免疫荧光专用玻片
SHI-FIX™ coverslips
-
产品编号： SB-Shifix25
-
相关CAS号：
-
分子式：
-
分子量：
| 订货号 | 规格 | 价格 | 库存 | 数量 | 购买 | |
|---|---|---|---|---|---|---|
| SB-Shifix25 | 25片/盒 | 1800.00元 | 10 |
|
细胞免疫荧光主要用于蛋白定位研究，相互作用研究和细胞信号转导研究。细胞免疫荧光就是将免疫学方法和荧光标记技术相结合，研究特异性抗原在细胞内的分布。细胞免疫荧光特性高，敏感性强，速度快，主要原理也是利用抗原抗体反映抗原抗体结合后再用荧光标记，通过显微镜下观察来确定某种特异性抗原是否存在。
根据培养的细胞类型，可分为贴壁细胞和悬浮细胞（淋巴细胞，血液的白细胞）。贴壁细胞有天然贴壁的属性，而悬浮细胞不依赖支持物表面，在培养液中呈悬浮状态生长。
免疫荧光实验的基础前提就是让细胞能固定在玻片上。悬浮细胞如何贴壁或者固定到玻片上，是全世界科研人员面对的一个难题。传统上，或用细胞离心甩片机制备细胞片或直接涂片法制备细胞涂片，然后把细胞片于乙醇或丙酮或多聚甲醛固定。无论甩片还是涂片，都是黑盒子实验。每个人操作手法不一样，即使同一个人操作，每一批实验，也都没法评价细胞片上的细胞固定的情况，而这一步至关重要。盲目而又没底的只能硬着头皮往下做。浪费的不仅是时间，还有抗体等很多试剂。如何解决这些痛点？悬浮细胞免疫荧光专用玻片Shi-Fix Coverslips带来终极解决方案！
您是否希望悬浮细胞的免疫荧光像贴壁细胞一样容易？现在就是这样！
Shi-fix™玻片允许悬浮细胞分层或直接作为单层生长。简单，无忧的实验方案，无需离心即可将悬浮细胞固定到载玻片上。只需将细胞添加到载玻片上，等待30分钟，用PBS洗涤未结合的细胞并继续免疫染色（或继续培养以获得所需的细胞密度）。
这些玻片也可用于染色悬浮细胞以进行显微镜检查，或用于固定悬浮细胞以进行共聚焦显微镜检查。更重要的是，如果需要，您还可以在细胞附着在玻片上时刺激它们。您可以像贴壁细胞一样处理非贴壁细胞！
Do you wish suspension cell Immunofluorescence was as easy as that of adherent cells?
Now it is! Our innovative Shi-fix™ cover-slips or multi well plates allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining.Or continue the culture to obtain desired cell densities for suspension cell immunofluorescence.
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so.
产品订购信息：
使用该产品的部分文献：
1. Genetic Disruption of Transfer RNA Modifications in Human Cancer. Laia Coll San Martin. Universitat De Barcelona, Facultat De Medicina Doctoral Thesis 2021
2. Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody. Xiaoyong Chen, Benedette J. Cuffari, Valentina Dubljevic, et al. ImmunoHorizons June 1, 2022, 6 (6) 356-365; DOI: https://doi.org/10.4049/immunohorizons.2100091
3. Coming in and Finding Out: Blending Receptor-Targeted Delivery and Efficient Endosomal Escape in a Novel Bio-Responsive siRNA Delivery System for Gene Knockdown in Pulmonary T Cells. Kandil R, Xie Y, Heermann R, et al. Adv Ther (Weinh). 2019;2(7):1900047. doi:10.1002/adtp.201900047
4. Specific Targeting of Somatostatin Receptor Subtype-2 for Fluorescence-Guided Surgery Servando Hernandez Vargas et al. Clin Cancer Res July 15 2019 25 (14) 4332-4342; DOI:10.1158/1078-0432.CCR-18-3312
5. Carboxy-terminal dendrimers with phenylalanine for a pH-sensitive delivery system into immune cells including T cells. Hiroya Shiba et al. 2022 Apr 6;10(14):2463-2470. doi: 10.1039/d1tb01980e.
6. Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Jeffrey R. Whiteaker and Tao Wang et al. Cancers 2021, 13(15), 3843; https://doi.org/10.3390/cancers13153843
Shi-Fix说明书：
Shi-fix™ Coverslips 产品说明书下载
1. Wash cells with PBS and resuspend 2-5 million cells per ml in PBS
2. Place the Shi-fix™ cover-slips on a 12-well ELISA plate for ease of handling. Add the cells directly to Shi-fix™ cover-slips.(0.2-0.3 million cells per coverslip).
3. Let the cells settle and attach for 30 mins. Attachment for some cell types may improve by incubating longer but do not exceed 1 hour. Wash gently with 2ml
PBS to remove unbound cells (Do not pipet PBS straight onto the coverslips. Add PBS to the sides of the plate well followed by gentle rocking for 3-5 mins).
4. Check for cell attachment on a microscope. If further culturing is needed, add 1ml medium otherwise proceed to fixation, permeabilization and
immunostaining.
点击化学铜离子配体THPTA三(3-羟丙基三唑甲基)胺
760952-88-3
PerkinElmer小动物活体成像底物D-荧光素钾盐
115144-35-9
