紫外线可切生物素化叠氮
UV Cleavable Biotin-Azide
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产品编号: EVU102
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相关CAS号:N/A
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分子式:N/A
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分子量:781.92
产品简介
The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
* Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
* Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
* Also available: 13C6-UV-Biotin-azide(EVU151)
产品详情
产品类型/Product Type: | Small Molecule |
产品名称/Name: | UV Cleavable Biotin-Azide |
化学式/Chemical Formula: | C38H51N7O9S |
分子量/Molecular Weight: | 781.92 |
外观形态/Format: | Solid |
溶解性/Solubility: | DMSO, MeOH |
光解/ Photolysis: | λ=365nm |
来源/Source: | Synthetic |
存储温度/Storage: | Upon receipt store at -20C |
运输温度/Shipped: | Ambient temperature |
参考文献
1. Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman, K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.; Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
2. An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-Derived Electrophiles by Streptavidin Catch and Photo-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.; Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
3. Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC. Global, in situ, site-specific analysis of protein S-sulfenylation. Nat Protoc. 2015 Jul;10(7):1022-37. 文献链接
4. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. 文献链接
细胞点击化学反应流程示意图